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Gentamicin protection assay to assess bacterial internalization in the human EDMs
Day 0: Plate cells in 24-well trans-well
Day1: Media change
Day2: Infect the EDMs with E.coli for 1–3 h then add gentamicin to kill extracellular bacteria. Lyse the cells and prepare the bacteria dilutions and plate
Day3: Count the bacteria and replate the dilutions if needed
