Preparation of primary culture media (100 mL) (Stored at 4°C for 1–2 months according to the expire date)
| Reagent | Final concentration | Amount (mL) |
|---|---|---|
| Adv. DMEM-F12 | NA | 79 |
| Fetal bovine serum (FBS) | 20% | 20 |
| Glutamax 100× (200 mM) | 2mM | 1 |
| Total | 100 |
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