Components | Stock conc. | Volume | Final conc. |
---|---|---|---|
50% CM | 49 mL | ||
Y-27632 (ROCKi) | 10 mM | 50 μL | 10 μM |
SB431542 | 10 mM | 50 μL | 10 μM |
Glutamax | 200 mM (100×) | 250 μL | 1 mM |
N-acetyl cysteine (NAC) | 500 mM | 100 μL | 1 mM |
rh-EGF | 100 μg/mL | 25 μL | 50 ng/mL |
Nicotinamide | 1M | 500 μL | 10 mM |
A-83-01 | 2 mM | 12.5 μL | 500 nM |
SB202190 | 30 mM | 17 μL | 10 μM |