Fig. 1.
Oligomerisation of EspB is promoted by an acidic environment. (A) Size exclusion chromatography profiles of M. tuberculosis EspB2-358 at 210 μM in 20 mM acetate buffer (pH 5.5), 150 mM NaCl and 20 mM Tris (pH 8.0), 150 mM NaCl. Void volume corresponds to 0.8 mL elution volume. (B) Oligomer/monomer ratios at different protein concentrations in conditions from panel (A). The absorbance values of the oligomer were taken at 1.14 mL while the monomer values were at 1.48 mL. (C–D) Presence of the different oligomer species from M. tuberculosis EspB2-348 at pH 5.5 and pH 8.5 obtained by native mass spectrometry.