Fig. 2.
Impact of EspB C-terminus processing on oligomerisation. (A) Scheme of the different constructs used in this work, where EspB2-460 is in blue, EspB2-358 in orange (MycP1 cleavage site), EspB2-348 in grey, EspB2-287 in yellow and EspB7-278 in green. Structural model from PDB ID 4XXX, while the C-terminal region is a representation of an unfolded protein. Arrows represent the end of each construct. Melting temperatures were obtained following the dependency of the circular dichroism signal as a function of temperature. (B) Size exclusion chromatograms of each construct corresponding to the colours in panel (A), resulting from 50 μL sample injection at 220 μM eluted in 20 mM acetate buffer (pH 5.5), 150 mM NaCl. Void volume corresponds to 0.8 mL elution volume.