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Loss of the EspB preferential orientation by removal of its C-terminal residues. (A–B) Representative micrograph of EspB2-348 with preferential orientation at 0°-tilt angle or 40°-tilt angle. (C) Representative micrograph of EspB2-287 with random orientation taken at 0°-tilt angle. Insets correspond to the respective 2D classes. Scale bars in A–C represent 50 nm; scale bars in insets represent 5 nm.
