Fig. 6.

Evaluation of EspB pore. (A–B) Electrostatic potential of EspB oligomer at pH 5.4 and (B) neutral pH 6.7 (−10 kT/e − red, 10 kT/e − blue). The protonation state was assigned by PROPKA (Olsson et al., 2011) and electrostatic calculations were generated by APBS (Baker et al., 2001) and PDBPQR (Dolinsky et al., 2004). (C) Surface representation of amino acid hydrophobicity according to the Kyte-Doolittle scale (polar residues – purple, non-polar residues – gold). (D) High-resolution 2D class of EspB heptamer with extra density in the middle. (E) Projection of the 3D map obtained for the 7 ​+ ​1 EspB oligomer. (F) C1 3D map of 7 ​+ ​1 EspB oligomer with local symmetry applied to the heptamer ring. (G) C1 3D map of the 7 ​+ ​1 EspB oligomer with 8-fold local symmetry applied and models fitted to the map.