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Relative expression levels of the GnRH and RFRP genes in hypothalamus of hens. Relative mRNA expression levels were normalized with β-actin mRNA. Data represent mean ± SEM. Bars with different lowercase letters were significantly different (P < 0.05). The difference between the same letters was not significant (P > 0.05). Bars without superscript was not significant (P > 0.05). GnRH, gonadotropin-releasing hormone; RFRP, RFamide-related peptides.
