Table 1.

Primer of genes for QPCR.

Primers	NCBI NO	Sequences (5′-3′)	Tm (°C)	Product size(bp)
GnRH-F	NC_052553.1	CGGGAAGAGTTGGAGCGATT	60.11	142
GnRH-R		CGGGAAGAGTTGGAGCGATT	60.03
RFRP-F	NC_052533.1	GGCCGAGTGCTTATTTGCCT	59.75	177
RFRP-R		CTTCCCGAATCTCTGTGGCA	59.38
GnRHR-F	NC_052541.1	GGTACTGGTTCTGTCCACGA	59.04	108
GnRHR-R		CATAGGTGATGGGGTCCAGG	59.23
RFRPR-F	NC_052537.1	CAATGGCAGCTGGGCTAATG	59.61	151
RFRPR-R		CAGGATGTTGCCGATCATGC	59.69
FSHR-F	NC_052534.1	GCCTCTGTGAAGGCAGGATA	59.17	135
FSHR-R		CTGTGAAAGCTCCCTTCGGA	59.68
LHR-F	NC_052534.1	GGGCTTTCCCAAGCCTACAT	60.03	133
LHR-R		TGGTGTCTTTATTGGCGGCT	59.96
PRLR-F	NC_052572.1	AGGAGTTACAGCCTGGGATGA	60.27	124
PRLR-R		TCACATCAAGGGCTCACGAAA	59.93
ESR1-F	NC_052534.1	GCGACATGTACGTGGAAAGC	59.91	145
ESR1-R		AGGCTGCTTGACCCAAAAGA	59.81
KITLG-F	NC_052532.1	AGCGCTGCCATTCCTTATGA	59.82	121
KITLG-R		ATCTGTCACTGGATTCCCGC	59.82
CYP11A1-F	NC_052541.1	CGTGGACACGACTTCCATGA	59.96	175
CYP11A1-R		GAGAGTCTCCTTGATGGCGG	60.04
β-Actin-F	NC_052545.1	CATTGTCCACCGCAAATGCT	59.76	108
β-Actin-R		AGCCATGCCAATCTCGTCTT	59.75