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. 2021 Jul 13;12:660488. doi: 10.3389/fimmu.2021.660488

Figure 2.

Figure 2

HA restricts T cell mobility and inhibits the antitumor effect of CAR-T cells. (A) Schematic diagram of the structures of CAR gene expression cassettes. (B) Schematic diagram of the Transwell tumor killing coculture system. (C) Results of killing assays performed with anti-MSLN CAR-T cells targeting the gastric cancer cell lines BGC823GL and KATOIIIGL in the Transwell coculture system; error bars denote the S. D, and the results were compared with two-way ANOVA. **P < 0.01. (D) GFP fluorescence images of viable BGC823GL cells at the endpoint of the killing assay at each E:T ratio. (E) Concentrations of IFN-γ and (F) Granzyme B in the supernatant of Transwell chambers at each E:T ratio determined by ELISA; error bars denote the S. D, and the results were compared with an unpaired t test. **P < 0.01; ***P < 0.001.