Figure 7.

In vitro binding assays confirm direct interaction between RseX and (A, B). fimB (-46 to +83 with respect to translational start) and (C, D). ihfB (-53 to +70 with respect to translational start). 1.3 pmol RseX was included in each 12 μL binding reaction. Dissociation constants (K_d) as shown in B/D were calculated using the modified Hill equations (Ryder et al., 2008). Notably, both targets have lower K_d values than previously reported target ompA ( Supplementary Figure 5 ), suggesting stronger interactions for fimB and ihfB.