Fig. 4.
Ablation of Srsf3 in the neural crest lineage results in a midline facial clefting phenotype. (A-F′) Gross morphology of E10.5 (A-B″), E12.5 (C-D′) and E14.5 (E-F′) Srsf3fl/fl;Wnt1-Cre+/+ control embryos (top) and Srsf3fl/fl;Wnt1-Cre+/Tg cKO embryos (bottom) viewed either laterally before (A-F) and after (A′,B′) DAPI staining (white) or frontally after DAPI staining (white) (A″,B″,C′-F′). Dotted black lines (A,B) outline nasal pits and PA 1. fb, forebrain; mb, midbrain; LNP, lateral nasal process; MxP, maxillary process; MdP, mandibular process; MNP, medial nasal process; FNR, frontonasal region; MxR, maxillary region; MdR, mandibular region. (G-H″″) Lateral (G,H), dorsal (G′,H′) and ventral (G″,H″) views of craniofacial skeletal preparations, dissected mandibles (G‴,H‴), and dissected hyoid bones and thyroid, cricoid and tracheal cartilages (G″″,H″″) generated from E18.5 control embryos (G-G″″) and cKO embryos (H-H″″). n≥3 biological replicates per genotype per timepoint. na, nasal; fmx, frontal process of maxilla; zpmx, zygomatic process of maxilla; fr, frontal; jg, jugal; sq, squamosal; pr, parietal; rtp, retrotympanic process; ip, interparietal; so, supraoccipital; pmx, premaxilla; mx, maxilla; crp, coronoid process; lo, lamina obturans; zps, zygomatic process of squamosal; MC, Meckel's cartilage; etm, ectotympanic; fpx, frontal process of premaxilla; ppmx, palatal process of maxilla; pppl, palatal process of palatine; pl, palatine; ptg, pterygoid; at, ala temporalis; bs, basisphenoid; sp, processus styloidius. Scale bars: 1 mm.