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. 2021 Jul 13;15:696440. doi: 10.3389/fnins.2021.696440

FIGURE 1.

FIGURE 1

AS69 does not affect PFF uptake, but reduces early pathology. (A) Representative images of primary neurons acquired 24 or 72 h after treatment with either PFF or BSA, and in addition either AS69 or BSA. Neurons were stained for AS69 (inset with white border, not included in merged image), phospho-aSyn (red border inset and red channel in merged image), human aSyn (green border inset and green channel), and neuronal markers [microtubule-associated protein 2 (MAP2) + βIII-tubulin, blue border inset and blue channel]. Scale bar: 10 μm. (B) Quantification of the area positive for human aSyn within the area delineated by the neuronal markers in neurons as depicted in panel (A) (n = 4 independent preparations, ≥10 neurons per experimental group, p = 0.007 for PFF + BSA vs. PFF + AS69 at 72 h, two-way ANOVA followed by Bonferroni post hoc test). (C) Quantification of phospho-aSyn within the same neurons as in panel (B) (p = 0.009 for PFF + BSA vs. PFF + AS69 at 72 h, two-way ANOVA followed by Bonferroni post hoc test). All markers represent the mean of all technical replicates of one individual preparation; lines represent mean ± SD of independent preparations. **p < 0.01.