Figure 8.

Interaction of neutrophils with heat-killed (HK) yeast cells. (A) Neutrophils with HK-C. albicans (Nɸ+HK-CA), neutrophils with HK-C. glabrata (Nɸ+HK-CG), and neutrophils with HK-S. cerevisiae (Nɸ+HK-SC) were observed at 3 h post infection. Neutrophils’ cell membrane sialic acids were stained with WGA, tetramethylrhodamine conjugate (red), nucleic acids stained with DAPI (blue), and each yeast cell was stained with Oregon Green (green). White arrows indicate clusters of phagocytized yeasts. Images are representative of sets of different experiments and were captured at 63× magnification. Scale bars represents 10 μm. (B) CA-HK, CG-HK, and SC-HK were incubated with NETs-enriched supernatants during 3 h. Samples were run on a 1% GreenSafe agarose gel to evaluate yeasts ability to digest nucleic acids and NETs (representative image of a set of different experiments).