FIGURE 6.

Human milk modifies epithelial cell protein expression and basolateral secretion. (A,B) Volcano plots of differential protein abundance (high false discovery rate) in the basolateral culture supernatant of 2PD monolayers NT or treated for 48 h with 20% HM (v/v) or IF (w/v). Data represent protein secreted by up to three monolayers per condition. Red dots indicate HM unique proteins; blue dots indicate epithelial cell-derived proteins; green dots indicate proteins derived from both HM and epithelial cells. (C) Protein-protein interaction analysis of 61 upregulated proteins produced by HM-treated monolayers selected based on the cut off shown in panel (A). High confidence interaction score = 0.700. Color line indicates types of protein-protein interactions based on co-expression (black line), co-occurrence (blue line), experimentally determined (magenta line), curated database (light blue line) and textmining search (green line). (D) Enrichment analysis of GO terms annotated for cellular component, molecular function, and biological process of the 61 upregulated proteins as described in panel (A).