Table 4.
The most significant enriched terms (potential function and pathway of target genes) based on biological process GO† enrichment (white rows) and KEGG‡ pathway (gray rows) of the miRNAs associated with achalasia.
| miRNA | Enriched Term | Target genes | A p-value* |
|---|---|---|---|
| hsa-miR-217 | Nonsmall cell lung cancer- Homo sapiens- hsa05223 | E2F3;KRAS;FOX3;FHIT | 0.004 |
| Endometrial cancer- Homo sapiens- hsa05213 | TCF7L2;PTEN;KRAS;FOXO3 | 0.004 | |
| Negative regulation of cell aging (GO:0090344) | PTEN;SIRT1;MARCH5 | 0.011 | |
| Cellular response to oxidative stress (GO:0034599) | NR4A2;TP53INP1;FOXO3;SIRT1;HIF1A;EZH2 | 0.011 | |
| Prostate cancer- Homo sapiens-hsa05215 | TCF7L2;PTEN;E2F3;KRAS | 0.016 | |
| Regulation of myelination (GO:0031641) | TCF7L2;PTEN;TNFRSF21 | 0.041 | |
| hsa-miR-216b-5p | Melanoma- Homo sapiens- hsa05218 | CDK6;CDK4;MAPK1;KRAS;FGF10 | 0.034 |
| Pathways in cancer-Homo sapiens-hsa05200 | CDK6;FZD5;TPM3;CDK4;COL4A4;FZD9;TCEB2;MAPK1;KRAS;FGF10 | 0.036 | |
| Signaling pathways regulating pluripotency of stem cells-Homo sapiens_hsa04550 | SMAD1;FZD5;FZD9;MAPK1;LHX5;KRAS | 0.036 | |
| Nonsmall cell lung cancer-Homo sapiens-hsa05223 | CDK6;CDK4;MAPK1;KRAS | 0.037 | |
| hsa-miR-215-5p | Cell cycle-Homo sapiens-hsa04110 | RB1;CDKN2D;CDKN2A;BUB1B;CDC7;TTK;CDC14A;ANAPC10;CDC20;ORC4;ORC1;CCNE1;RAD21;MCM3;MCM6;MAD2L1 | 0.012 |
| hsa-miR-143-3p | Glioma-Homo sapiens- hsa05214 | PDGFRA;MDM2;AKT1;MAPK1;BRAF;CALM3;KRAS;HRAS;IGF1R | -1.84E-05 |
| Sphingolipid signaling pathway- Homo sapiens- hsa04071 | CERS4;SGPL1;SPTLC2;PPP2R5E;BCL2;AKT1;MAPK1;KRAS;TNF;HRAS | -6.69E-05 | |
| MicroRNAs in cancer- Homo sapiens-has 05206 | TRIM71;PDGFRA;DNMT3A;PTGS2;MAPK7;ERBB3;FSCN1;MDM2;BCL2;MAPK1;KRAS;HRAS;CD44 | 0.0007 | |
| Nonsmall cell lung Cancer-Homo sapiens-hsa05223 | AKT1; MAPK1; BRAF; KRAS; FHIT; HRAS | 0.0012 | |
| Colorectal Cancer-Homo sapiens-hsa05210 | SMAD3; BCL2; AKT1; MAPK1; BRAF; KRAS | 0.0015 | |
| Bladder Cancer-Homo sapiens-hsa05219 | MDM2; MAPK1; BRAF; KRAS; HRAS | 0.0019 | |
| Regulation of neuron death (GO:1901214) | ERBB3;UBE2V2;BCL2;AKT1;XIAP;KRAS;BRAF;HRAS;TNF;BB | 0.004 | |
| Regulation of neuron apoptotic process (GO:0043523) | ERBB3;UBE2V2;BCL2;XIAP;KRAS;BRAF;HRAS;TNF;BBC3 | 0.004 | |
| Negative regulation of neuron death (GO:1901215) | ERBB3;UBE2V2;BCL2;AKT1;XIAP;KRAS;BRAF;HRAS | 0.006 | |
| Immune response regulating cell surface receptor signaling pathway GO:0002768) | PDGFRA;NCKAP1;PLEKHA1;YWHAB;LIMK1;ERBB | 0.0080 | |
| hsa-miR-6507-5p | cytokinesis_(GO:0000910) | RACGAP1;PRC1;NEK7;MYH9;CEP55;RHOB | 0.033 |
| hsa-miR-135a-5p | Jak STAT signaling pathway-Homo sapiens-hsa04630 | PIAS4;MYC;MPL;BCL2;STAT6;JAK2 | 0.002 |
| Signaling pathways regulating pluripotency of stem cells-Homo sapiens-hsa04550 | BMPR2;APC;MYC;JAK2;SMAD5;SKIL | 0.002 | |
| Colorectal cancer-Homo sapiens-hsa05210 | APC;MYC;BCL2;BIRC5 | 0.004 | |
| MicroRNAs in cancer-Homo sapiens-hsa05206 | MARCKS;BMPR2;APC;ROCK1;MYC;BCL2;IRS2 | 0.004 | |
| TGF beta signaling pathway-Homo sapiens-hsa04350 | BMPR2;ROCK1;MYC;SMAD5 | 0.008 | |
| Cellular response to BMP stimulus (GO:0071773) | HEYL;GATA6;SMAD5 | 0.031 | |
| Response to BMP (GO:0071772) | HEYL;GATA6;SMAD5 | 0.031 | |
| neuron_projection_regeneration_(GO:0031102) | BCL2;APOA1;JAK2 | 0.031 | |
| Axon development (GO:0061564) | BCL2;APOA1;JAK2 | 0.031 | |
| Axon regeneration (GO:0031103) | BCL2;APOA1;JAK2 | 0.031 | |
| Positive regulation of ntrinsic apoptotic signaling pathway (GO:2001244 | PIAS4;SIAH1;BCL2;SKIL | 0.031 | |
| hsa-miR-3609 | Pathways in cancer-Homo sapiens-hsa05200 | ITGB1;EGLN3;PRKCB;F2R;FZD9;XIAP;HIF1A;IGF1R;TGFBR2;BMP2;CCND1;MDM2;MAPK1;CRK;APPL1;F2RL3 | 0.020 |
| Proteoglycans in cancer Homo sapiens hsa05205 | ITGB1;CCND1;PRKCB;CAV1;FZD9;MDM2;RRAS2;MAPK1;HIF1A;THBS1;IGF1R | 0.020 | |
| Endocytosis-Homo sapiens hsa04144 | SH3GLB1;HSPA8;RAB5B;RAB4A;ZFYVE9;SH3KBP1;CAV1;F2R;EPS15L1;IGF1R;TGFBR2;RAB11FIP1;MDM2 | 0.020 | |
| Focal adhesion- Homo sapiens-hsa04510 | RAP1B;ITGB1;CCND1;PRKCB;CAV1;XIAP;PAK6;MAPK1;CRK;THBS1;IGF1R | 0.020 | |
| hsa-miR-194-5p | Adherens junction- Homo sapiens-hsa04520 | TJP1;EP300;RAC1;IGF1R | 0.048 |
| Proteoglycans in cancer-Homo sapiens-hsa05205 | CAV1;FZD6;RAC1;HBEGF;IGF1R | 0.049 | |
| Focal adhesion- Homo sapiens-hsa04510 | CAV1;TLN2;RAC1;IGF1R;ITGA9 | 0.049 | |
| HIF-1 signaling pathway-Homo sapiens-hsa04066 | CDKN1B;EP300;RBX1;IGF1R | 0.049 |
†GO, Gene Ontology; ‡KEGG, Kyoto Encyclopedia of Genes and Genomes; *A p-value, Adjusted p-value.