Table.
The list of studies conducted on autophagy and DNA repair systems.
| Cell line/tissue and organism | Drug/genetic modification | Repair mechanism | DNA repair-associated target | Autophagy status | Quantification of DNA damage | Reference |
|---|---|---|---|---|---|---|
| Ampk -/- and WT MEFs | UVB | NER | XPC | N.D. | Slot blot assay of CPD and 6-4PP | (Wu et al., 2013) |
| Atg5 -/- ; Atg7 -/- and WT MEFs, HaCaT | UVB | NER | XPC | Inhibited | Slot blot assay of CPD and 6-4PP | (Qiang et al., 2016) |
| Hs294T, A2058 | Cisplatin | NER | XPA | Activated | PARP1 activity | (Ge et al., 2016) |
| Primary human fibroblast | XPA-/- | NER | XPA, PARP1 | Inhibited | PARP1 activity | (Fang et al., 2014) |
| Parp1 -/- and WT MEFs, MCF7 | Starvation | BER | PARP1 | Inhibited | PARP1 activity | (Rodríguez-Vargas et al., 2016) |
| HL1 mouse cardiomyocyte | Serum and Glucose deprivation | BER | OGG1 | Activated | Detection of γH2AX, p-ATM, NBS1 and 8-oxoG | (Siggens et al., 2012) |
| MLE-12 | Hyperoxia | BER | OGG1 | Inhibited | Comet tail length, OGG1 activity | (Ye et al., 2017) |
| U2OS | 5-FU | BER | MSH2 | Activated | PARP1 activity | (SenGupta et al., 2013) |
| C. elegans | 5-FU | BER | MSH2, MSH6 | Activated | RPA-1 filament formation, CHK-1 phosphorylation | (SenGupta et al., 2013) |
| AGS, NCI-N87 | 5-FU, AT101 | BER | APE1 | Activated | N.D. | (Wei et al., 2016) |
| HCT116, HEC59 | 6-thioguanine (6-TG) | MMR | MLH1, MSH2 | Activated | PARP1 activity | (Zeng et al., 2007) |
| HCT116 | 6-thioguanine (6-TG) and 5-fluorouracil (5-FU) | MMR | MLH1 | Activated | CHK-1 phosphorylation | (Zeng et al., 2013) |
| CAOV-3 | Cisplatin | HR | BRCA2 | Activated | Comet tail length | (Wan et al., 2018) |
| 786-O | Sunitinib | HR | RAD51 | Activated | Micronuclei formation | (Yan et al., 2017) |
| Mouse Oocytes | Rad51 RNAi | HR | RAD51 | Activated | Comet tail length | (Kim et al., 2016) |
| CNE-1, CNE-2 | Ionizing radiation (IR) | HR | RAD51 | Activated | Detection of γH2AX | (Mo et al., 2014) |
| HT-29, DLD-1 | Ionizing radiation (IR) | NHEJ | UVRAG | Activated | Detection of γH2AX, nuclear foci positivity of 53BP1 | (Park et al., 2014) |
| TLR4mut liver | Diethylnitrosamine (DEN) | NHEJ | Ku70 | Inhibited | Detection of γH2AX and 8-oxoG | (Wang et al., 2013) |
| Sqstm1 -/- and WT MEFs | Ionizing radiation (IR) | NHEJ | FLNA and RAD51 | Activated | Detection of γH2AX and TP53BP1 | (Hewitt et al., 2016) |
| Bone marrow, Hematopoietic cells | Ionizing radiation (IR) | HR, NHEJ | N.D. | Activated | Detection of γH2AX, Comet tail length | (Lin et al., 2015) |
| L2A -/-; Atg7 -/- and WT MEFs | Etoposide | HR, NHEJ | CHK1 | Inhibited | Detection of γH2AX, Comet tail length | (Park et al., 2015) |
| Atg7-/- and WT MEFs | Ionizing radiation (IR) | HR, NHEJ | CHK1 | Activated | Detection of γH2AX, Comet tail length, Plasmid-based NHEJ and HR assay | (Liu et al., 2015) |