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. 2021 Jun 23;45(3):301–313. doi: 10.3906/biy-2101-50

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Copyright © 2021 The Author(s)

This article is distributed under the terms of the Creative Commons Attribution License ( http://creativecommons.org/licenses/by/4.0/ ), which permits unrestricted use and redistribution provided that the original author and source are credited.

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The microphotographs of the morphological and immunohistochemical experiments. a. A microscopic focus showing minimal differentiation of osteosarcoma cells characterized by osteoid matrix production (hematoxylin-eosin staining; 400). b. Osteosarcoma cells displaying diffuse cytoplasmic staining with BMP2 antibody, similar to the striated muscle bundles on the right lower corner of the frame (immunohistochemistry staining; 100). c. Osteosarcoma cells displaying score 12 nuclear and cytoplasmic staining patterns with p27 (immunohistochemistry staining; 100). d. 1 (+) staining intensity similar to bronchial epithelium with caspase-3 displayed in a metastatic focus in lung tissue (Immune histochemistry staining; 40).