Table 2.
Primer sequences used in RT-PCR.
| Protein | Gene | Forward primer | Reverse primer |
|---|---|---|---|
| Bone morphogenic protein 2 | BMP2 | ACCCGCTGTCTTCTAGTGTTG | TTCTTCGTGATGGAAGCTGAG |
| Bone morphogenic protein 4 | BMP4 | ACTTCGAGGCGACACTTCTG | GTCCACCTGCTCCCGAAATA |
| Collagen type 1, alpha 1 | COL1A1 | AAGAGGAAGGCCAAGTCGAG | TAAGACAGCTGGGGAGCAAA |
| Osteocalcin (BGLAP) | OCN | CCTGACTGCATTCTGCCTCT | TCGTCACAATTGGGGTTGAG |
| Osteopontin (SPP1) | OPN | CCGATGAATCTGATGAGTCCTT | TCCAGCTGACTTGACTCATGG |
| Platelet factor 4 (CXCL4) | PF4 | AGCCCTGAGCTGCTTCTTCT | TCCTGCTTTGATCACCTCCA |
| Actin, beta | ActB | TTCTACAATGAGCTGCGTGTG | GGGGTGTTGAAGGTCTCAAA |