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Proposed delivery mode by pHLIP-PNA conjugates (A) the transmembrane section of pHLIP interacting with lipid bilayer, (B) low surface pH leads to partial protonation of negative residues triggering interfacial helix formation and deeper partitioning into lipid bilayer, and (C) the transmembrane helix formation and release of PNA into cytosol by disulfide cleavage.
