Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Templated fluorogenic detection of oligonucleotides using two PNAs. (A) Templated FRET depends on hybridization of PNAs to adjacent positions on the target sequence to bring the donor and acceptor in proximity. Templated reactions such as (B) Staudinger reaction or (C) conjugate addition of thiols can be used to turn on fluorescence of a caged pro-fluorophore. (D) Photochemical templated reactions target an immolative linker which both tethers and quenches a pro-fluorescent molecule.
