Skip to main content
. 2021 Jul 13;13:684171. doi: 10.3389/fnagi.2021.684171

FIGURE 1.

FIGURE 1

Amlexanox (AMX) reduces the phosphorylation of Tbk1 targets STING and p62 upon Stab Wound Injury (SWI). Western blots of cortical biopsies (at 7 dpi) homogenates show trend toward decreased levels of phospho STING (A,B) but an increase in total STING levels (A,C), resulting in a significant decrease in the fraction of phosphorylated STING (D). Likewise, levels of phospho p62 (E,F) were significantly decreased the AMX treatment (irrespective of SWI) whereas the total amount of p62 was slightly increased (E,G), resulting in a substantial decrease in the fraction of phosphorylated p62 in the overall p62 pool (H). Levels of phosphorylated Tbk1 were strongly increased in AMX-SWI samples (I,J) whereas total levels of Tbk1 were reduced in these samples (but slightly increased by AMX or SWI alone) (K), resulting in a massive increase in the fraction of phosphorylated Tbk1 in the Tbk1 pool in AMX-SWI samples (L). n = 3 animals/group. Average ± SEM; one-way ANOVA ns: not significant, ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, ****p < 0.0001; full statistical report in Supplementary Table 3; original full uncropped WB in Supplementary File 1.