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. 2021 Jul 13;13:684171. doi: 10.3389/fnagi.2021.684171

FIGURE 6.

FIGURE 6

Tbk1 blockade induces a distinct inflammatory cytokines profile upon Stab Wound Injury (SWI). qPCR from whole-biopsy homogenates were obtained from the injury site (or the corresponding anatomical location in sham mice) from animals treated with either vehicle or AMX at 7 dpi. AMX treatment after SWI significantly upregulated the expression of IL-33 (A), IFN-γ (H), IL-17 (I) and IL-19 (J). Interestingly, AMX after SWI (7 dpi) also significantly reduced the expression of IL-1β (B), IL-18 (D), IL-25 (E). Notably, AMX alone, irrespective of concomitant SWI, substantially increased the levels of IL-17 mRNA (I). No difference in expression levels was observed upon SWI with or without inhibitor treatment in IL6 (C), TNF alpha (F), IFN beta (G), IL10 (K), and IL12 (L). (n = 3 animals/group with 4 sections/animal. Average ± SD, plus individual data points indicated; two-way ANOVA with Tukey post hoc; ns: not significant, ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, ****p < 0.0001; full statistical report in Supplementary Table 8).