FIGURE 8.

Persistent effect of 7-days Tbk1 blockade on microglial DAM-like phenotype after 33 days of washout. (A,B) Mice were subject to cortical SWI injury and treated with AMX for 7 days; AMX was then withdrawn and mice were sacrificed 43 days later (40 days after injury). In AMX-treated mice, even after long wash-out, the density of CD11c⁺ and CD169⁺ microglia (TMEM119⁺) remained significantly higher than in vehicle-treated animals. (n = 3 animals/group with 4 sections/animal. Average ± SD, plus individual data points indicated; two-way ANOVA with Tukey post hoc; ns: not significant, ^∗p < 0.05, ^∗∗p < 0.01, ^∗∗∗p < 0.001, ^****P < 0.0001; full statistical report in Supplementary Table 10). Length of scale bars for both low-magnification and high-magnification insets is 200 μm.