FIGURE 9.

Tbk1 blockade enlarges the gliotic scar and amplifies the deposition of CPSG at 7dpi after Stab Wound Injury (SWI). (A,D) The density of reactive astrocytes (GFAP⁺) is substantially larger at 7 dpi in mice treated with AMX for 7 days after SWI compared to vehicle-treated controls. (B,E) The extent of the CSPG scar deposition (as revealed by immunostaining with the CS-56 antibody) is substantially increased by the treatment with AMX for 7 days after SWI injury. (C,F) The density of NeuN⁺ cells is unaffected by either vehicle or AMX treatment. Western blots of cortical biopsies (at 7 dpi) homogenates show decreased levels of (G,H) PSD-95 and (I,J) NR1. (n = 3 animals/group with 4 sections/animal. n = 4 animals for the vehicle treated group and n = 6 animals for the amlexanox treated group. Average ± SD; one-way ANOVA with Tukey post hoc; ns: not significant, ^∗p < 0.05, ^∗∗p < 0.01, ^∗∗∗p < 0.001, ^****P < 0.0001; original uncropped blots in Supplementary File 2; full statistical report in Supplementary Table 11). Length of scale bars for both low-magnification and high-magnification insets is 200 μm.