Figure 6.

Annexin V apoptosis assay following treatment with palbociclib, navitoclax, or combination of the two agents among the panel of HPV- head and neck squamous cell carcinoma (HNSCC) cell lines. Cells were exposed to 0.5 μM palbociclib, 1 μM navitoclax, or combination of the two agents, or 1 μM staurosporine (positive control). Cells were harvested after 72 hours and analyzed by flow cytometry. Representative flow cytometry analysis graphs (A, C, E) illustrate staining with 7-AAD and Annexin V-PE under each drug treatment condition for each line. Proportions of viable and apoptotic cells in each drug treatment condition for each line (B, D, F), with data displayed as mean ± standard deviation from three biological replicates performed on different days. Statistical significance is indicated: * if p < 0.05, ** if p < 0.01, comparing combination treatment group with each single agent all using two-tailed paired t-tests.