Figure 6: Proteomic Analysis of LUC7L2^KO and Galactose-Grown Cells Reveals Secondary Complexes I+III+IV Accumulation. See also Figure S6 and Table S8.

(A) Volcano plots and immunoblots of OXPHOS protein expression in LUC7L2^KO and (B) in galactose-grown K562 cells. > indicates a protein not shown but reported in Table S8. (C) Blue-Native PAGE on a mitochondria-rich fraction isolated from LUC7L2^KO K562 cells and stained with coomassie or (D) immunoblotted with the indicated antibodies. Parallel blots in which the same lysate was loaded were used to avoid antibodies cross-reactivity. SCs: supercomplexes. CI-V: complexes I to V. (E) Immunoblot on K562 cells expressing Cas9 and treated with sgRNAs targeting glycolytic enzymes with the indicated antibodies. (F) Model of the secondary regulation of the respiratory chain by LUC7L2 and galactose.