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. 2021 Jun 21;27(7):gaab040. doi: 10.1093/molehr/gaab040

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© The Author(s) 2021. Published by Oxford University Press on behalf of European Society of Human Reproduction and Embryology.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 3. — Gene expression patterns during oocyte maturation using oligo(dT) primers. Relative expression in cDNA samples synthesised using oligo(dT) primers in maturing bovine oocytes, as determined by quantitative RT-PCR without normalisation. (A) ACTB, (B) CCNA1, (C) CCNB1, (D) CCNB2, (E) CDK2, (F) EIF4A3, (G) GAPDH, (H) HRRT1, (I) RLP15, (J) SDHA, (K) YWHAZ. GV, GVBD, MI, MII, respectively refer to germinal vesicle, germinal vesicle breakdown, metaphase I and metaphase II stages. Absolute expression in GV oocytes is set at 1. Error bars represent standard deviation. Samples were collected from pools of 50 oocytes with three biological replicates.