Figure 4.

TFCH detects drug-induced carbonylation in live rat kidney slices. Schematic representation showing the region of kidney used for imaging (A). Representative photomicrographs assembled (stitched) from multiple sections of the renal cortex (B). As indicated, rat kidney slices were exposed to cisplatin (150 μg/mL), gentamicin (4.6 mg/mL), or vehicle (buffer; no drug) for 1 h, followed by the addition of TFCH (2 μM) for 30 min. The tissue samples were washed, fixed, and processed as described in the Methods before imaging. An inset (enhanced) (C) of the control (no drug) slice is showing the location of renal tubule (RT) and glomerulus (G). Scale bar, 200 μm