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. Author manuscript; available in PMC: 2021 Jul 27.
Published in final edited form as: J Leukoc Biol. 2020 Feb 4;108(3):867–882. doi: 10.1002/JLB.4MA0120-115RR

FIGURE 6. Inhibition of IL-10 production by CD73 is important for optimal production of reactive oxygen species by polymorphonuclear leukocytes (PMNs) in response to S. pneumoniae infection.

FIGURE 6

PMN were isolated from the bone marrow of the indicated strains of mice and left untreated (A and B) or treated for 30 min with the indicated rIL-10 (50 ng/ml) (C) or 100 μM Adenosine or anti-IL-10 (1 μg/ml JES5–2A5), or isotype control (1 μg/ml) (D). PMNs were then infected with S. pneumoniae pre-opsonized with homologous sera (+Sp) or treated with 3% matching sera (uninfected) and intracellular reactive oxygen species (ROS) production measured by chemiluminescence of luminol (A) or extracellular ROS production measured by chemiluminescence of isoluminol in the presence of HRP (B-D). Representative data are shown from one of six (B) and one of four (A, C-D) separate experiments with one mouse per strain per experiment where each condition is tested in quadruplicates. Significant differences (P < 0.05) were determined by 2-way ANOVA followed by Sidak’s multiple comparisons test