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. 2021 Jul 26;41(7):BSR20200767. doi: 10.1042/BSR20200767

Figure 2. NEAT1 directly targeted miR-98-5p.

Figure 2

(A) Sequences of miR-98-5p and the potential miR-98-5p-binding sites of NEAT1. Seed sequences and mutant are marked. (B) RNA-FISH tested NEAT1 in U251 cells by Olympus BX51 microscope, magnification 400×, bar = 10 μm. (C) RNA pulldown was performed to enrich miRNAs interacted with lncRNA NEAT1 in U251 cells. (D,E) Relative luciferase activity was performed by dual-luciferase reporter assay. (F,G) Effect of knockdown of NEAT1 on the expression of miR-98-5p in glioma cells. Relative expression levels of miR-98-5p were detected by qRT-PCR. **P<0.01 as compared with the control. The experiments were run three times.