Fig. 6.

Overexpression of LIMK1-EGFP increases hippocampal P-cofilin in 3-month old APP/PS1 mice. A Sample Western blots of hippocampal protein lysate showing protein levels of LIMK1, total cofilin and P-cofilin in APP/PS1 mice expressing LIMK1-EGFP or EGFP. B Summary graph showing significantly increased hippocampal LIMK1 protein level in APP/PS1 mice expressing LIMK1-EGFP compared to WT mice expressing EGFP (APP/PS1 + EGFP n = 5 independent experiments from 5 mice, APP/PS1 + LIMK1-EGFP n = 5 independent experiments from 5 mice, p = 0.004, two-tailed t-test). C Summary graph showing no significant difference in total cofilin protein level between APP/PS1 mice expressing EGFP and LIMK1-EGFP (APP/PS1 + EGFP n = 5 independent experiments from 5 mice, APP/PS1 + LIMK1-EGFP n = 5 independent experiments from 5 mice, p = 0.442, two-tailed t-test). D Summary graph showing significantly increased P-cofilin protein level in hippocampus in APP/PS1 mice expressing LIMK1-EGFP compared to WT mice expressing EGFP (APP/PS1 + EGFP n = 5 independent experiments from 5 mice, APP/PS1 + LIMK1-EGFP n = 5 independent experiments, p = 0.002, two-tailed t-test). Data are presented as mean ± s.e.m. **p < 0.01, n = not significant