Fig. 7.

Overexpression of LIMK1-EGFP in the hippocampus has no effect on LTP in 3-month old WT mice. A Input–output curves of fEPSP showing no difference between WT mice expressing EGFP and LIMK1-EGFP (WT + EGFP n = 5 slices from 5 mice, WT + LIMK1-EGFP n = 6 slices from 6 mice; genotype: F_(1,3) = 4.626, p = 0.121; stimulus intensity: F_(8,24) = 24.095, p < 0.001; repeated two-way ANOVA). B Paired pulse ratio showing no differences between WT mice expressing EGFP and LIMK1-EGFP (WT + EGFP n = 6 slices from 5 mice, WT + LIMK1-EGFP n = 6 slices from 6 mice; genotype: F_(1,4) = 0.292, p = 0.617; inter-pulse interval: F_(7,28) = 14.844, p < 0.001; repeated two-way ANOVA). Scale bars: 0.2 mV/10 ms. C TBS induced comparable LTP at the CA1 synapse in WT mice expressing EGFP or LIMK1-EGFP. D Summary graph showing no significant difference in LTP of last 10 min between WT expressing EGFP and LIMK1-EGFP (WT + EGFP n = 8 slices from 5 mice, WT + LIMK1-EGFP n = 9 slices from 5 mice, p = 0.308, two-tailed t-test). Data are presented as mean ± s.e.m. ns = not significant