Table 1.

Cohorts, number of animals and housing conditions for all behavior experiments

Background strain	Sex	N	Behavior test	Housing	Figure
Neonatal mice
B6	Male Female	WT n = 17; Spred1-/- n = 12 WT n = 7; Spred1-/- n = 10	USV at P4-12 Righting reflex P4, P8 (first subgroup only, 12–17/genotype)	With birth litter and dam	Figure 2, Additional file 1: Fig. 3A, B
B6	Spred1+/− Female dams for the above neonatal cohort	Naïve n = 7; experienced n = 5	Note: for experienced dams—2nd litter n = 3; 3rd litter n = 1; 4th litter n = 1
Adult mice
B6;129T2 F2	Female Male	WT n = 8; Spred1-/- n = 8 WT n = 8; Spred1-/- n = 8	Tube test at 9–13 weeks	Couples: WT with Spred1-/-	Figure 1A, B
B6;129T2 F2	Male Female	WT n = 6; Spred1-/- n = 7 WT n = 10; Spred1-/- n = 7	Open field at 6–15 weeks	Mixed genotype group housing	Additional file 1: Fig. 3C, D
B6	Male	WT n = 10; Spred1+/−  n = 10	Tube test at 10–15 weeks	Couples: WT with Spred1+/−	Additional file 1: Fig. 1D–F
B6	Female	WT n = 10; Spred1-/-  n = 10	Tube test at 11–20 weeks nesting at 14–23 weeks	Couples: WT with Spred1-/-	Additional file 1: Fig. 1A–C Figure 1G
B6	Female	WT n = 7; Spred1-/-  n = 9	Nesting at 20–35 weeks	Couples: WT with Spred1-/-	Figure 1G
B6	Female	WT n = 11; Spred1-/-  n = 11	Marble burying at 8–20 weeks	Mixed genotype group housing	Additional file 1: Fig. 2F
B6	Male	WT n = 15; Spred1-/- n = 14	Marble burying at 9–10 weeks (n = 8/genotype only) USV at 10–11 weeks	Couples: WT with Spred1-/-	Additional file 1: Fig. 2F Figure 1C–F
B6	Male	WT n = 9; Spred1-/- n = 9	Resident intruder at 10–13 weeks	Mixed genotype group housing	Additional file 1: Fig. 1G–H
B6	Female	WT n = 15; Spred1-/- n = 15	Three-chamber test at 20 weeks (n = 13–14/genotype) burrowing at 28 weeks (n = 8/genotype only) MEK inhibitor + nesting at 40 weeks. (WT + DMSO n = 8; WT + PD325901 n = 7; Spred1-/- + DMSO n = 7; Spred1-/- + PD325901 n = 8)	Mixed genotype group housing	Additional file 1: Fig. 2A–E Additional file 1: Fig. 2G Figure 4
MEK inhibitor + Automated tube test
B6: WT + DMSO vs Spred1-/- + DMSO	Male	WT + DMSO n = 4; Spred1-/- + DMSO n = 4	Tube test at 11–18 weeks	Couples: WT + DMSO with Spred1-/- + DMSO	Additional file 1: Fig. 4A–C
B6: WT + DMSO vs Spred1-/- + DMSO	Female	WT + DMSO n = 4; Spred1-/- + DMSO n = 4	Tube test at 10–11 weeks	Couples: WT with Spred1-/-	Additional file 1: Fig. 4D–F
B6: WT + DMSO vs Spred1-/- + PD325901	Female	WT + DMSO n = 8; Spred1-/- + PD325901 n = 8	Tube test at 10–12 weeks (cohort1), 23 weeks (cohort2)	Couples: WT + DMSO with Spred1-/- + PD325901	Figure 3A; Additional file 1: Fig. 5A
B6: WT + DMSO vs WT + PD325901	Female	WT + DMSO n = 8; WT + PD325901 n = 8	Tube test at 11–15 weeks	Couples: WT + DMSO with WT + PD325901	Figure 3C; Additional file 1: Fig. 5C
B6: Spred1-/- + DMSO vs Spred1-/- + PD325901	Male	Spred1-/- + DMSO n = 6; Spred1-/- + PD325901 n = 6	Tube test at 10–18 weeks	Couples: Spred1-/- + DMSO with Spred1-/- + PD325901	Figure 3B; Additional file 1: Fig. 5B