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. 2021 Jul 27;21:397. doi: 10.1186/s12935-021-02098-1

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 2 — IGHG1 modulation influenced tumor cell proliferative capabilities of gastric cancer cells. A Western Blot analysis on IGHG1 protein expression level differences in gastric cancer cell lines (MKN45, HGC27, MGC-803, BGC-823, AGS, MKN28) and normal gastric epithelial cell line GES-1; B qRT-PCR detection of IGHG1 mRNA expression in gastric cancer cell lines (MKN45, HGC27, MGC-803, BGC-823, AGS, MKN28) and normal gastric epithelial cell line GES-1; C Design and transfection of IGHG1 specific shRNA and IGHG1 overexpression vector into AGS1 and MKN45 cell line respectively; D qRT-PCR analysis on the modulative effects of IGHG1 specific shRNA and overexpression vector on IGHG1 mRNA expression in AGS and MKN45 cell line; E CCK8 assay on AGS1 and MKN45 cellular proliferative capabilities, each cell group was respectively transfected with IGHG1 specific shRNA or IGHG1 overexpression vectors; F Edu assay on AGS1 and MKN45 cellular proliferative capabilities, each cell group was respectively transfected with IGHG1 specific shRNA or IGHG1 overexpression vectors; G, H. Western Blot and qRT-PCR analysis on cyclinD1 and PCNA protein and mRNA expression level in cell groups respectively transfected by IGHG1 specific shRNA and overexpression vectors