Fig. 2.

Specific podocyte Sirt1 conditional knockout mice were generated. Identification of podocyte primary culture cell morphology (a, left) and WT-1 (a, middle) and synaptopodin (a, right) expression by immunofluorescence. Sirt1 mRNA level by qRT-PCR (b). SIRT1 protein levels in podocyte primary culture cells from cKO mice and WT controls determined by Western blot (c). Measurement of urine albumin levels (albumin-to-Cr ratio) (d), PAS staining (e), and WT-1 expression between WT and cKO mice (f). Results are presented as means ± SEM (n = 6). *p < 0.05 versus the WT group. SIRT1, silent mating type information regulation 2 homolog 1; WT, wild type; cKO, conditional knockout; PAS, periodic acid-Schiff.