Fig. 4.

Podocyte conditional Sirt1 knockout aggravated Aldo-induced podocyte injury. qRT-PCR analysis of nephrin and podocin in the kidney cortex (a) and Western blotting analysis for nephrin and podocin (b). Upper: representative immunoblots. Lower: densitometric analysis. Electron microscopy of podocyte foot (c). Results are presented as means ± SEM (n = 6). *p < 0.05 versus the WT + sham group. #p < 0.05 versus WT + Aldo. Sirt1, silent mating type information regulation 2 homolog 1; WT, wild type.