Fig. 5.

Loss of Sirt1 in podocyte activated NLRP3 inflammation. Mice were treated with a continuous infusion of Aldo for 14 days. NLRP3 mRNA level by qRT-PCR analysis (a), ELISA analysis of urine IL-18 levels (b), and representative immunoblots of Western blotting analysis for NLRP3 and caspase-1 (c) and densitometric analysis (d). Results are presented as means ± SEM (n = 6). *p < 0.05 versus the WT + sham group. #p < 0.05 versus WT + Aldo. Sirt1, silent mating type information regulation 2 homolog 1; WT, wild type; NLRP3, NLR family pyrin domain containing 3.