Fig. 7.

SIRT1 overexpression inhibited NLRP3 inflammation activation in podocyte. Cells were grown on 6-well plates until 60–70% confluence, and cultured podocytes were treated with different concentrations of aldosterone (50, 100, and 200 nM). qRT-PCR analysis of Sirt1 (a). Cells were grown on 6-well plates until 50–60% confluence, transfected with Sirt1 or vehi plasmid for 24 h, and then, treated with Aldo (200 nmol/L) for another 24 h. Representative immunoblots of Western blotting analysis of overexpressed SIRT1 and densitometric analysis (b), NLRP3 mRNA level (c), ELISA analysis of IL-18 levels in the medium (d), representative immunoblots of NLRP3 and caspase-1 protein levels (e), and densitometric analysis (f). Results are presented as means ± SEM (n = 4). *p < 0.05 versus the vehi group. #p < 0.05 versus the Aldo group. SIRT1, silent mating type information regulation 2 homolog 1; NLRP3, NLR family pyrin domain containing 3; IL-18, interleukin-18.