Fig. 8.

Sirt1 overexpression inhibited MtD and podocyte injury. Cells were grown on 6-well plates until 50–60% confluence, transfected with Sirt1 or vehi plasmid for 24 h, and then, treated with Aldo (200 nmol/L) for another 24 h. mtDNA copy number (a) and quantitation of JC-1 fluorescence by flow cytometry (b). Western blotting analysis of podocin and nephrin and densitometric analysis (c). Results are presented as means ± SEM (n = 4). *p < 0.05 versus the vehi group. #p < 0.01 versus the Aldo group. Sirt1, silent mating type information regulation 2 homolog 1; mtDNA, mitochondrial DNA; MtD, mitochondrial dysfunction.