. Author manuscript; available in PMC: 2021 Jul 27.

Published in final edited form as: Stem Cell Res. 2021 Apr 15;53:102354. doi: 10.1016/j.scr.2021.102354

Table 2.

Characterization and validation.

Classification	Test		Result	Data
Morphology	Photography		All iPSC lines are morphologically normal	Not shown; available from authors
Phenotype	Qualitative analysis	Confirmed expression of pluripotency markers: OCT4, NANOG, and SOX2		Fig. 1 panel A
	Quantitative analysis	Assessed % of positive cells for pluripotency marker, OCT3/4 by immunocytochemistry counting and cell surface markers, SSEA-4 by flow cytometry. All lines are more that 94% positive for both markers.		Fig. 1 panel B
Genotype	Karyotype (G-banding) and resolution	1. 46XX, Resolution 425–500		Supplementary panel A
		2. 46XX, Resolution 350–450
		3. 46XX, Resolution 400–500
		4. 46XX, Resolution 400–450
		5. 46XX, Resolution 425–550
		6. 46XX, Resolution 425–475
		7. 46XX, Resolution 425–500
		8. 46XX, Resolution 400–425
		9. 46XX, Resolution 475–550
		10. 46XX, Resolution 425–500
		11. 46XX, Resolution 475–550
		12. 46XX, Resolution 375–450
		13. 46XX, Resolution 425–500
		14. 46XX, Resolution 450–475
		15. 46XX, Resolution 400–525
Identity	Microsatellite PCR (mPCR) OR STR analysis	STR analysis 16 specific loci were tested. All isogenic lines matched 100%.		Supplementary data 1 Submitted in archive with journal
Mutation analysis (IF APPLICABLE)	Sequencing	1. I2020T PL2F4-homozygous		Fig. 1 panel C
		2. I2020T PL2D7-heterozygous
		3. G2019S PL2A9-homozygous
		4. G2019S PL2H7-heterozygous
		5. Y1699C PL3H2-homozygous
		6. Y1699C PL2F7-heterozygous
		7. R1441C PL2F5-homozygous
		8. R1441C PL3C2-heterozygous
		9. N1437H PL3D7-homozygous
		10. N1437H PL3F5-heterozygous
		11. K1906M PL2H7-homozygous
		12. T1348N PL1B6-homozygous
		13. KO PL1C4-c.5694_5697delTGAA (p.Tyr1898Ter) and c.4016_4985del (p.Ile1339LysfsX12)
		14. KO PL1C6-c.4015_4018delATTG (p.Ile1339TrpfsX15) and c.4016_4985del (p.Ile1339LysfsX12)
	Southern Blot OR WGS	Not performed		N/A
Microbiology and virology	Mycoplasma	Mycoplasma testing was done by RT-PCR. All clones-negative		Supplementary Fig. 1
Differentiation potential	e.g. Embryoid body formation OR Teratoma formation OR Scorecard OR Directed differentiation	The STEMdiff™ Trilineage Differentiation Kit (StemCell) was used to test differentiation potential.
Differentiation potential		We confirmed the expression of endoderm (AFP, SOX-17), mesoderm (<-SMA, Brachyury) and ectoderm (Nestin, MAP2) markers in all clones.		Fig. 1 panel D
Donor screening (OPTIONAL)	HIV 1 + 2 Hepatitis B, Hepatitis C	Not performed		N/A
Genotype additional info (OPTIONAL)	Blood group genotyping	Not performed		N/A
Genotype additional info (OPTIONAL)	HLA tissue typing	Not performed		N/A