Figure 4.
In vitro therapeutic study of RA/TPPP-Lip. (A) Relative cell viabilities of HCT116 cells treated with Empty Liposome, Free TPPP, TPPP-Lip+Laser, Free RA-XII, RA-Lip, and RA/TPPP-Lip+Laser for 24h. *P< 0.05, **P < 0.01. (B) Relative cell viabilities of HCT116 cells treated with different concentrations of RA/TPPP-Lip without or with 660 nm laser irradiation. **P < 0.01, ***P < 0.001. (C) Representative images of HCT116 cells co-stained by Calcein-AM (green) and PI (red) after treatment with PBS (control), RA-Lip, TPPP-Lip with 660 nm laser irradiation, and RA/TPPP-Lip with 660 nm laser irradiation. Data represent mean ± s.d. (n = 3). Scale bar: 20 μm.