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. 2021 Jul 13;12:679836. doi: 10.3389/fimmu.2021.679836

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Copyright © 2021 Mishto, Rodriguez-Hernandez, Neefjes, Urlaub and Liepe

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Quantification of KRAS_5-6/8-14 G12V cis-spliced and KRAS_5-13 G12V non-spliced peptides in in vitro digestion kinetics. (A, B) MS extracted ion chromatograms of the target peptides in the in vitro digestion kinetics of the “MF” (A) and “MG” (B) series (0 h and 4 h) and of the mix of the two synthetic peptides. (C, D) Quantification of the MS ion peak area of the KRAS_5-6/8-14 G12V cis-spliced and KRAS_5-13 G12V non-spliced peptides in the in vitro digestion kinetics of the “MF” (C) and “MG” (D) series. In (A–D) in vitro digestion kinetics of the synthetic polypeptide KRAS_2-35 G12V with purified proteasomes using either HMDA (“MF” series) or TMDA (“MG” series) are shown. Samples have been measured through Q Exactive Orbitrap (one technical replicate each condition). The MS ion chromatograms correspond to the m/z = 421.275 – 421.875.