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. 2021 Jul 13;12:679836. doi: 10.3389/fimmu.2021.679836

Figure 5.

Figure 5

MS2 spectra of the KRAS5-6/8-14 G12V cis-spliced peptide. MS2 spectrum of the peptide KRAS5-6/8-14 G12V [KL][VVGAVGV] identified in an in vitro 20 h digestion of the synthetic polypeptide KRAS2-35 G12V with proteasomes and of the cognate synthetic peptide. The in vitro 20h digestion was performed months earlier than the assays shown in Figures 3–5 of (11). This in vitro 20 h digestion and the cognate synthetic peptide were measured through the Fusion Lumos Orbitrap months earlier than the assays shown in Figures 4 and 5 of (11). The peptide sequence is shown with the corresponding b-, a- and y-ions identified. The G12V mutation is depicted in bold. In the spectra, assigned peaks for b-, a- and y-ions are reported in red. Ion neutral loss of ammonia is symbolized by * and double charges by ++.