FIGURE 5.

Predicted spike drug inhibitors screened using a VSV-SARS2 infection assay reveals two promising hits. (A) Individual drugs from the library set were used at 10 μM to treat GFP reporter viruses, VSV-SARS2 and VSV, for 30 min prior to infection of Vero cells at 0.5 MOI or 0.1 MOI respectively. The infection media was replaced with fresh media at 1 h post-infection and fluorescent reporter values were measured the next day. (B) Half-maximal inhibitory concentration (IC₅₀) curves and values were obtained for Imatinib (compound 20) and Lapatinib (compound 19) using the same VSV-SARS infection assay performed for library screening. All data were normalized as percent infection or inhibition for drug-treated conditions vs. no-treatment control. The values are means, with error bars displaying standard deviation between the triplicate wells.