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. 2021 Jun 22;17(10):2606–2621. doi: 10.7150/ijbs.60292

Figure 7.

Figure 7

Knockdown of MEG3 reversed the suppression of DDP on tumor metastasis ability and abolished the activation of NLRP3/caspase-1/GSDMD pathway. A) H&E staining of breast tumor specimens in xenograft mice. Blue arrows indicated the tumor cells with pyroptosis-like changes. B) H&E staining of important organs including lung, bone, liver, brain, kidney, and heart. The blue arrow showed the metastatic tumor cells. The three-line table showed the lung metastasis incidence in the xenograft model. C) Immunohistochemical staining of Ki67, NLRP3, caspase-1, GSDMD, IL-18, and IL-1β expression in breast tumor specimens of xenograft mice. D) Western blot analysis of NLRP3, caspase-1, GSDMD, IL-18, and IL-1β in breast tumor specimens of xenograft mice. GAPDH was included as a loading control. The dose of DDP in the above-mentioned in vivo experiments was 5 mg/kg twice a week and continued to 24 days. Data are shown as mean ± SD (n=8). *P<0.05; **P<0.01; ***P<0.001. Scale bar, 50 µm.