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. 2021 Jun 16;17(10):2504–2522. doi: 10.7150/ijbs.51638

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Up-regulated miR-214-3p identified in human osteosarcoma cells and tissues promotes proliferation and migration, and inhibits apoptosis of osteosarcoma cells. miR-214-3p expression in paired osteosarcoma and paracancerous normal tissues from 5 osteosarcoma patients (A, left panel), in the osteosarcoma cells (143B, U-2 OS, Saos-2, MG-63 and MNNG) and the normal human osteoblast hFOB1.19 cells (A, right panel) determined by RT-qPCR assay. RT-qPCR analysis validated successfully over-expressed (AgomiR-214-3p) or silenced (AntagomiR-214-3p) miR-214-3p in 143B (B) and U-2 OS (C) cells, following phenotypes were then evaluated: (D) viability by xCELLigence RTCA DP system monitor, (E) self-renew ability with colony formation assay, (F) migration by Transwell assay and (G) apoptosis by flow cytometric assay. *p < 0.05, **p < 0.01, ***p < 0.001.

Up-regulated miR-214-3p identified in human osteosarcoma cells and tissues promotes proliferation and migration, and inhibits apoptosis of osteosarcoma cells. miR-214-3p expression in paired osteosarcoma and paracancerous normal tissues from 5 osteosarcoma patients (A, left panel), in the osteosarcoma cells (143B, U-2 OS, Saos-2, MG-63 and MNNG) and the normal human osteoblast hFOB1.19 cells (A, right panel) determined by RT-qPCR assay. RT-qPCR analysis validated successfully over-expressed (AgomiR-214-3p) or silenced (AntagomiR-214-3p) miR-214-3p in 143B (B) and U-2 OS (C) cells, following phenotypes were then evaluated: (D) viability by xCELLigence RTCA DP system monitor, (E) self-renew ability with colony formation assay, (F) migration by Transwell assay and (G) apoptosis by flow cytometric assay. *p < 0.05, **p < 0.01, ***p < 0.001.