Skip to main content
. 2021 Jun 16;17(10):2504–2522. doi: 10.7150/ijbs.51638

Figure 2.

Figure 2

Figure 2

miR-214-3p promotes proliferation and metastasis of osteosarcoma cells by degrading DKK3 to activate Wnt/β-catenin signaling. (A) Diagram of potential complementary sequence of miR-214-3p in DKK3 3 -'UTR (http://www.targetscan.org/). (B) Expressions of DKK3 mRNA in paired osteosarcoma (Tumor) and paracancerous normal tissues (Control) from 3 patients detected by RT-qPCR. (C) Overall survival curves of osteosarcoma patients with Log-rank (Mantel-Cox) test. Expression levels of DKK3 in osteosarcoma cells (143B, U-2 OS, Saos-2 and MNNG) and human osteoblast cell hFOB1.19 determined by RT-qPCR (D) and Western blot (E). (F) DKK3, β-catenin and LEF1 expressions in 143B cells after knockdown (AntagomiR-214-3p) or over-expression (AgomiR-214-3p) of miR-214-3p determined with RT-qPCR assay. (G) DKK3, active-β-catenin and LEF1 expressions in 143B cells after knockdown (AntagomiR-214-3p) or over-expression (AgomiR-214-3p) of miR-214-3p identified by Western blot assay. *p < 0.05, **p < 0.01, ***p < 0.001.