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. 2021 Jun 16;17(10):2504–2522. doi: 10.7150/ijbs.51638

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Cantharidin inhibits proliferation and metastasis of osteosarcoma cells via down-regulating miR-214-3p. (A) Cantharidin dose dependently inhibited miR-214-3p expression in 143B and U-2 OS cells. To further investigate direct interaction of miR-214-3p in cantharidin inhibited osteosarcoma cell proliferation and metastasis: miR-214-3p was either over-expressed or silenced in 143B and U-2 OS cells before exposure to 0 (DMSO in PBS: 1/10,000 V/V) or 2.5 μM cantharidin, followed by investigating self-renew ability with colony formation assay (B and C); cell viability (D and E), cell migration (F and G) and cell invasion (H and I) analyzed by xCELLigence RTCA DP system. Statistics assay for Figure 3F-I have been performed by analyzing the data at the time point of 24 h. NS. not statistically significant.*p < 0.05, **p < 0.01, ***p < 0.001.

Cantharidin inhibits proliferation and metastasis of osteosarcoma cells via down-regulating miR-214-3p. (A) Cantharidin dose dependently inhibited miR-214-3p expression in 143B and U-2 OS cells. To further investigate direct interaction of miR-214-3p in cantharidin inhibited osteosarcoma cell proliferation and metastasis: miR-214-3p was either over-expressed or silenced in 143B and U-2 OS cells before exposure to 0 (DMSO in PBS: 1/10,000 V/V) or 2.5 μM cantharidin, followed by investigating self-renew ability with colony formation assay (B and C); cell viability (D and E), cell migration (F and G) and cell invasion (H and I) analyzed by xCELLigence RTCA DP system. Statistics assay for Figure 3F-I have been performed by analyzing the data at the time point of 24 h. NS. not statistically significant.*p < 0.05, **p < 0.01, ***p < 0.001.