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. 2021 Jun 16;17(10):2504–2522. doi: 10.7150/ijbs.51638

Figure 5.

Figure 5

Figure 5

Figure 5

Figure 5

Cantharidin promotes cell cycle arrest and apoptosis in 143B and U-2 OS cells measured with flow cytometer and Western blot. (A-E) Dose-dependently (0, 1.25, 2.5 and 5 μM) triggered apoptosis of 143B and U-2 OS cells: (A) apoptosis rate of 143B cells; (B) representative diagrams of apoptotic 143B cells; (C) apoptotic rate of U-2 OS cells; (D) representative diagrams of apoptotic U-2 OS cells; (E) expressions of key proteins related to apoptosis. (F-J) Cell cycles of 143B and U-2 OS cells were arrested at M phase: (F and G) specific phase distribution and representative diagrams through cell cycle of 143B cells; (H and I) specific phase distribution and representative diagrams through cell cycle of U-2 OS cells; (J) expressions of cell cycle related key proteins identified by Western blot assay. β-actin served as a loading control for Western blot assay. *p < 0.05, **p < 0.01, ***p < 0.001.